Culturing and visualizing cells

Culturing and visualizing cells-test

1、单选题:
 The most direct source of monoclonal antibodies are ________ cells.‎‎‎
选项:
A: B lymphocytes
B: T lymphocytes
C: myeloma
D: Hybridoma
答案: 【 Hybridoma

2、单选题:
The fluorescent properties of dyes such as FITC can provide information on the​​​‌​
选项:
A: location of specific proteins.
B: concentration of  ions in specific regions of the cell.
C: the amount of  DNA in a cell.
D: volume of a cell.
答案: 【 location of specific proteins.

3、单选题:
What factors necessary for growth of animal cells in culture are provided by serum? ‏
选项:
A: amino acids
B: precursors of DNA synthesis
C: growth factors
D: vitamins
答案: 【 growth factors

4、单选题:
What is the advantage of using GFP-tag to visualize specific proteins, instead of staining cells with fluorescent dyes that bind to the target protein?‌‌‏‌
选项:
A: GFP fusions enable one to track changes in the location of the protein in living cells; staining usually requires fixed cells
B: GFP fusions enable higher resolution than staining with fluorescent probes.
C: GFP permits the position of the protein in the cell more precisely than fluorescent probes
D: GFP fusions are not subject to artifacts; fluorescent probes may introduce background artifacts.

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