大学MOOC 细胞生物学(厦门大学)1002485011 最新慕课完整章节测试答案

pre-test

1、单选题：
‎which one is NOT the typical morphological characteristics of apoptotic cells?‎
选项：
A: cell shrinkage
B: plasma membrane rupture
C: blebbing
D: apoptotic body formation
答案: 【 plasma membrane rupture】

What you must know about cellular senescence

1、单选题：
​Which one is NOT the morphological characteristics of cellular senescence?​
选项：
A: increased cell size
B: flat, enlarged cytomorphology
C: SA-β-GAL positive staining
D: apoptotic body formation
答案: 【 apoptotic body formation】

Cellular Energetics

1、单选题：
‎Where does glycolysis takes place?‍
选项：
A: Mitochondrial matrix
B: Cytosol
C: Mitochondrial intermembrane space
D: Mitochondrial inner membrane
E: Mitochondrial outer membrane
答案: 【 Cytosol】

2、单选题：
​Where is the electron transport chain found in plant cells?‌
选项：
A: Stroma of chloroplasts
B: Inner membrane of mitochondria
C: Thylakoid membranes of chloroplasts
D: Cytoplasm
答案: 【 Thylakoid membranes of chloroplasts】

3、单选题：
‍Energy released by the electron transport chain is used to pump H+ ions into which location?‌
选项：
A: Mitochondrial matrix
B: Mitochondrial intermembrane space
C: Mitochondrial inner membrane
D: Cytosol
答案: 【 Mitochondrial intermembrane space】

4、单选题：
​Inside an active mitochondrion, most electrons follow which pathway?‏
选项：
A: Glycolysis --> NADH --> oxidative phosphorylation --> ATP --> oxygen
B: Citric acid cycle --> FADH2 --> electron transport chain --> ATP
C: Pyruvate --> citric acid cycle --> ATP --> NADH --> oxygen
D: Citric acid cycle --> NADH --> electron transport chain --> oxygen
答案: 【 Citric acid cycle --> NADH --> electron transport chain --> oxygen】

5、单选题：
‏Which do the catabolic products of fatty acid breakdown enter into the citric acid cycle?‏
选项：
A: Pyruvate
B: Acetyl CoA
C: Succinyl CoA
D: Malate or fumarate
答案: 【 Acetyl CoA】

6、单选题：
​Brown-fat mitochondria uncouple oxidative phosphorylation to produce__________‏
选项：
A: ADP
B: Fat
C: Heat
D: Oxygen
答案: 【 Heat】

7、判断题：
‎During ATP synthesis, protons move “down” their electrochemical gradient through  the F0 complex of ATP synthase‎
选项：
A: 正确
B: 错误
答案: 【 正确】

8、判断题：
‎The outer mitochondrial membrane is derived from the bacterial plasma membrane​
选项：
A: 正确
B: 错误
答案: 【 错误】

9、填空题：
‏__________ is a lipid soluble molecule that acts to shuttle electrons within the mitochondrial inner membrane​
答案: 【 CoQ】

10、填空题：
‌The energy released by the passage of electrons along the respiratory chain is stored as an___________ across the inner mitochondrial membrane.‏
答案: 【 proton motive force】

Culturing and visualizing cells-test

1、单选题：
 The most direct source of monoclonal antibodies are ________ cells.‎‎‎
选项：
A: B lymphocytes
B: T lymphocytes
C: myeloma
D: Hybridoma
答案: 【 Hybridoma】

2、单选题：
The fluorescent properties of dyes such as FITC can provide information on the‎‎‎‎‎
选项：
A: location of specific proteins.
B: concentration of  ions in specific regions of the cell.
C: the amount of  DNA in a cell.
D: volume of a cell.
答案: 【 location of specific proteins.】

3、单选题：
What factors necessary for growth of animal cells in culture are provided by serum? ‏
选项：
A: amino acids
B: precursors of DNA synthesis
C: growth factors
D: vitamins
答案: 【 growth factors 】

4、单选题：
What is the advantage of using GFP-tag to visualize specific proteins, instead of staining cells with fluorescent dyes that bind to the target protein?‌‌‎‌
选项：
A: GFP fusions enable one to track changes in the location of the protein in living cells; staining usually requires fixed cells
B: GFP fusions enable higher resolution than staining with fluorescent probes.
C: GFP permits the position of the protein in the cell more precisely than fluorescent probes
D: GFP fusions are not subject to artifacts; fluorescent probes may introduce background artifacts.
答案: 【 GFP fusions enable one to track changes in the location of the protein in living cells; staining usually requires fixed cells】

5、单选题：
​Which one cleaves pre-miRNA in the cytoplasm to produce miRNA?‏​‏
选项：
A: Dicer
B: RISC
C: shRNA
D: siRNA
答案: 【